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Preface:
Two hundred and sixteen individual chapters dedicated to different cytometric
methodologies were published since 1990 in the six cytometry-committed volumes
of the series of Methods in Cell Biology (MCB; Volumes 33, 41, 42, 63, 64, and 75).
The editors of these volumes attempted to assemble chapters describing the most
widely used methods of flow- and quantitative image-cytometry. The chapters
outlined principles of these methods, their applications, advantages, as well as
possible pitfalls in their use, and presented them to the forum of cell biologists.
Within the series of MCB, these volumes received wide readership, high citation
rates, and were valuable in promoting cytometric techniques among cell biologists
across different fields.
The exceptionally high interest in the MCB chapters on cytometry prompted the
Publisher to propose a special edition of the ?Essential Cytometry Methods? within
the framework of the new series of volumes defined ?Reliable Lab Solutions.? This
volume presents the chapters describing the most frequently used methods among
those presented in the previous volumes. The chapters were selected based on high
frequency of citations and relevance of the methodology. Since most these methods
are still widely used, such an edition is contemporary and will be of use to many,
particularly to young investigators who are starting to use the methods of
cytometry in their research.
Authors of this volume were asked to update their chapters by providing a short
foreword to the original text and make corrections in the text, if needed. The
update highlights in brief progress in the methodology, novel reagents, new applications,
and sister methodologies developed since the original publication. Additional
references essential for the presentation of the update are included. Because
some authors, particularly of the chapters early published, in MCB volumes 33, 41,
and 42, could not be reached, their chapters remain without the update. Some
chapters were updated by the Editors who are familiar with the methodology
described in them.
Applications of cytometric methods have had a tremendous impact on research
in various fields of cell and molecular biology, immunology, microbiology, and
medicine. We hope that this volume will be of help to many researchers who need
these methods in their investigation, stimulate application of the methodology in
new areas, and promote further progress in science.
Zbigniew Darzynkiewicz
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Contact: Zbigniew Darzynkiewicz |