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Table of Contents:
1. Principles of Confocal Microscopy
J. Paul Robinson, Jennifer Sturgis, and Bartek Rajwa
2. Protein Labeling with Fluorescent Probes
Kevin L. Holmes and Larry M. Lantz
3. Cytometry of Fluorescence Resonance Energy Transfer
Gyorgy Vereb, Janos Matko, and Janos Szollosi
4. The Rainbow of Fluorescent Proteins
David W. Galbraith
5. Labeling Cellular Targets with Semiconductor Quantum Dot Conjugates
Kary L. Oakleaf, Michael S. Janes, Xingyong Wu, and Marcel P. Bruchez
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6. High-Gradient Magnetic Cell Sorting
Andreas Radbruch, Andreas Grutzkau, Birgit Mechtold, Andreas Thiel,
Stefan Miltenyi, and Eckhard Pfluger
7. Multiplexed Microsphere Assays (MMAs) for Protein and
DNA Binding Reactions
Kathryn L. Kellar and Kerry G. Oliver
8. Biohazard Sorting
Ingrid Schmid, Mario Roederer, Richard A. Koup, David Ambrozak,
Stephen P. Perfetto, and Kevin L. Holmes
9. Guidelines for the Presentation of Flow Cytometric Data
Mario Roederer, Zbigniew Darzynkiewicz, and David R. Parks
10. Detergent and Proteolytic Enzyme-Based Techniques for
Nuclear Isolation and DNA Content Analysis
Lars L. Vindeløv and Ib Jarle Christensen
11. DNA Content and Cell Cycle Analysis by the Propidium
Iodide-Hypotonic Citrate Method
Awtar Krishan and Ronald Hamelik
12. DNA Analysis from Paraffin-Embedded Blocks
David W. Hedley
13. Flow Cytometry and Sorting of Plant Protoplasts and Cells
David W. Galbraith
14. DNA Content Histogram and Cell-Cycle Analysis
Peter S. Rabinovitch
15. Simultaneous Analysis of Cellular RNA and DNA Content
Zbigniew Darzynkiewicz
16. Immunochemical Quantitation of Bromodeoxyuridine: Application to
Cell-Cycle Kinetics
Frank Dolbeare and Jules R. Selden
17. Cell-Cycle Kinetics Estimated by Analysis of Bromodeoxyuridine Incorporation
Nicholas H. A. Terry and R. Allen White
18. Flow Cytometric Analysis of Cell Division History Using Dilution
of Carboxyfluorescein Diacetate Succinimidyl Ester, a Stably
Integrated Fluorescent Probe
A. Bruce Lyons, Jhagvaral Hasbold, and Philip D. Hodgkin
19. Antibodies Against the Ki-67 Protein: Assessment of the Growth
Fraction and Tools for Cell Cycle Analysis
Elmar Endl, Christiane Hollmann, and Johannes Gerdes
20. Detection of DNA Damage in Individual Cells by Analysis of
Histone H2AX Phosphorylation
Peggy L. Olive
21. Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis
Zbigniew Darzynkiewicz, Xun Li, and Jianping Gong
22. Difficulties and Pitfalls in Analysis of Apoptosis
Zbigniew Darzynkiewicz, Elzbieta Bedner, and Frank Traganos
23. Cell Preparation for the Identification of Leukocytes
Carleton C. Stewart and Sigrid J. Stewart
24. Multicolor Immunophenotyping: Human Immune System Hematopoiesis
Brent Wood
25. Differential Diagnosis of T-Cell Lymphoproliferative Disorders by
Flow Cytometry Immunophenotyping. Correlation with Morphology
Wojciech Gorczyca and Sorina Tugulea
26. B Cell Immunophenotyping
Nicole Baumgarth
27. Telomere Length Measurements Using Fluorescence In Situ
Hybridization and Flow Cytometry
Gabriela M. Baerlocher and Peter M. Lansdorp
28. Sperm Chromatin Structure Assay: DNA Denaturability
Donald Evenson and Lorna Jost
29. Cell Membrane Potential Analysis
Howard M. Shapiro
30. Measurement of Intracellular pH
Michael J. Boyer and David W. Hedley
31. Intracellular Ionized Calcium
Carl H. June and Peter S. Rabinovitch
32. Oxidative Product Formation Analysis by Flow Cytometry
J. Paul Robinson, Wayne O. Carter, and Padma Kumar Narayanan
33. Phagocyte Function
Gregor Rothe and Mariam Klouche
34. Analysis of RNA Synthesis by Cytometry
Peter Østrup Jensen, Jacob Larsen, and Jørgen K. Larsen
35. Analysis of Mitochondria by Flow Cytometry
Martin Poot and Robert H. Pierce
36. Analysis of Platelets by Flow Cytometry
Kenneth A. Ault and Jane Mitchell
37. Detection of Specific Microorganisms in Environmental
Samples Using Flow Cytometry
Graham Vesey, Joe Narai, Nicholas Ashbolt, Keith Williams, and Duncan Veal
38. Flow Cytometric Analysis of Microorganisms
J. Paul Robinson
39. Flow Cytometry in Malaria Detection
Chris J. Janse and Philip H. Van Vianen
Zbigniew Darzynkiewicz
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Contact: Zbigniew Darzynkiewicz |